I have used a product from Cardinal Associates (877-359-3249) called Nim2. It is a 2 layer gradient for mouse and rat blood. I have used as little as 100ul of blood to get great yeild. The PBLs are left in much better shape, as lysing is very hard on them. Good luck, Steve McClellan BeckmanCoulter Cytometry
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:51 EST