Re: Mouse PBL and RBC lysis

From: Coulter Corporation (XENOMAN@erols.com)
Date: Tue Aug 17 1999 - 16:55:56 EST


I have used a product from Cardinal Associates (877-359-3249)
called Nim2.  It is a 2 layer gradient for mouse and rat blood.  I have
used as little as 100ul of blood to get great yeild.  The PBLs are left
in much better shape, as lysing is very hard on them.  Good luck,
Steve McClellan
BeckmanCoulter Cytometry



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