Hi again, Today's question: We're running an intracellular cytokine staining for lymphocytes and monocytes in parallel. So we start with whole blood, add our top-secret :) activators, and let that sit overnight. Then we plan to add BFA to "plug the hole" and capture any cytokines the cells are still making. The problem is, for lymphocytes this time frame is prob. ok (activation from 5pm to 9 am next morning) b/c it's the amount of time they need to gear up. But are Monocytes more rapid in their response? I'm afraid that if we do it this way by the time we add BFA our cells will not be making any more cytokines. Has anyone had the experience of overnight activation for purpose of intracellular staining the next morning? Any thoughts will be appreciated, Maciej S. Simm + Lab === `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3952 | `---------------------------------------------` _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com
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