Colleagues, A researcher here at UCONN would like to use flow to sort cells expressing the sapphire -GFP construct. The excitation profile appears to require a 406nm beam. I was wondering if anyone has experience with this GFP construct and could tell me what laser they are using to produce this line (HeCad?)? 2nd question, any thoughts, experiences with BD's new LSR tabletop analyser? Do you think an 8mw UV beam is sufficient for basic immunophenotyping utilizing cascade blue or Hoechst for DNA profiling? Thanks for your help. Gene Pizzo/UCONN Health
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