Flow Cytometry Group: We have had some trou\ble isolating dendritic cells from human tonsils. We tried negative selection with dynal beads, but got lousy purity--10%. We then tried to sort, using CD1a and CD21 to distinguish DC from FDC. We treated the tonsils with collagenase which supposedly increases the yield. It did, but the DC were all dead. Anyabody got a good protocol? Thanks. ron Ronald L. Rabin, M.D. rr84g@nih.gov
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