Hello Cytometrists, This is probably a boring old subject, but it's new for me: I have cells fixed in 4-5% formaldehyde, flow-sorted by some internal antibody label, and now I would like to quantify cellular protein contents, by various methods such as electrophoresis and HPLC. Can I do this from cells that were fixed with a protein cross-linker? I.e. can I get the proteins un-linked and without damage ? If yes, how ? Many thanks for any helpful tip, such as a reference or protocol. Ralph Bohmer, New England Medical Center
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