Hello everyone! I'm having serious problems with a user trying to get IL4 and IL5 to work either on Pharmingen HICK 2 cells, or human PBL. Is there anyone currently doing these here at Wash U, or in the city? She has tried working wity Mark Edinger at Pharmingen but really needs to be lead step by step through the assay, or I need to know if it cannot be done so I can tell her boss. Please, any help or suggestions for running 4 color (FACSCalibur) or the assay would be greatly APPRECIATED. please reply to jbragdon@pulmonary.wustl.edu Thanks, Jan Bragdon "If we knew what we were doing it wouldn't be called research, would it?" -- Albert Einstein
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