Dear flow users, I have questions regarding neutrophil priming/activation in rat peripheral blood. 1. How reliable is the up regulation of mac-1 (CD11b/CD18) as an activation marker of priming in flow cytometry? 2. Is flowcytometric analysis of the oxidative burst a reproducible measurement of neutrophil priming? Can anyone reccomend a kit. 3. What is the best agent to stimulate neutophils in vitro (PMA, fMLP)? Thankyou Dr Adam Bartlett Lecturer in Surgery Surgical Research Fellow The University of Auckland New Zealand e-mail a.bartlett@auckland.ac.nz (work) e-mail adambart@ihug.com (home) phone +64 9 3074949 X7805 (work) Home +64 9 3781903 Fax +64 9 3779656 Mobile 021 890894
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