Proteins of eosinophils' granules have extremely high affinity to bind FITC. FITC at concentration between 2-500 nM, thus, selectively labels eosinophils with minimal labeling of other blood cell types (Bedner et al., Cytometry 36: 77-82, 1999). Alternative methods of labeling of eosinophils also are discussed in this article. Zbigniew Darzynkiewicz
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