Differential Staining of DNA and RNA

From: Jeff Louie (jlouie@usc.edu)
Date: Wed Jul 14 1999 - 17:52:37 EST 

I am trying to locate a flow cytometry laboratory in the Los Angeles area
that can provide differential staining of DNA and RNA on cell culture
material using dyes such as acridine orange (AO) or a combination of
pyronin Y (PY) and Hoechst 33342 (PY-Hoeschst 33342). Our laboratory has a
MoFlo with appropriate lasers (argon and UV) to analyse this combination of
dyes but have no experience with this particular application. The PI that
wants the work done will pay for the service but has a tight deadline
(middle of August) to complete the work. I would apprecaite very much any
leads to labs doing this work or comments as to the difficulty of setting
it up in-house. Thank you for your response.

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