Hi Tina, I don't have OmniComp still, but I have FL-6 option which is possible to compensate with any fluorescence parameter derived from 1-st laser. Cy5 from PE-Cy5 is excitable from He-Ne and gets on APC detector in very small amount, perhaps 1%. I can compensate this when I put FL-6 plug on APC PMT but usually I don't. APC-Cy7 needed about 24% compensation with APC in my experiments. I thought OmniComp box can compensate everything (omnis!?), not only cross-beams. Regards, Sasha PS. Sorry, I am sending a message again. Server here was down the other day when I sent a message originally and I am not sure it reached you. Thanks. On Wed, 7 Jul 1999 16:38:02 -0500 Tina Rogers <Tina.Rogers@ccc.uab.edu> wrote: > > Greetings! > > I am now trying to figure out how to use Omnicomp for an 8 parameter/3 laser > analysis. > I have been told by BD that Omnicomp can only be used to subtract primary > (488) laser signals from secondary laser (HeNe) detectors. > At issue is cychrome vs. APC and PharRed. > Does anyone have a problem without the ability to subtract HeNe signals from > 488 detectors? > > Thanks in advance for any help > > TIna S. Rogers, Ph.D. > Manager, MAMDC FACS Core Facility > Division of Rheumatology > University of Alabama at Birmingham > 1900 University Blvd. > THT 405 > Birmingham, AL 35294-0006 > 205-934-1362 > ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606
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