Dear Carl, We had the same problem sometime ago and as you told, specially in the analysis of NOD/SCID mice transplanted with human cells. We solved the problem by using routinelly PI in the analysis to take out death cells and other debris DNA debris. So, we stablish a FSvsSSC window and then applied that window to PI(FL3 in our XL)vsFL4 and select the negative cells to PI. We add the PI just before the analysis at 2ug/ml concentration. In that way we obtain more clear dot-plots and histograms Good luck At 12:22 8/07/99 +0200, you wrote: > >Dear summer flowers, > >We have for some time cursed a phenomenon which we have referred to as the >"pölsa". The phenomenon occurs in isotype controls at various frequencies >depending on the type of material analysed, and leads to the formation of a >tail that stretches across the dotplot window. The problem seems to be most >frequent when analyzing chimeric NOD/SCID mice transplanted with human bone >marrow cells. >Does anyone have any solution to this or thoughts around how this should be >interpreted or possible be dealt with? > > > >Carl-Magnus Högerkorp >Stem cell laboratory >Department of Internal Medicine >University Hospital of Lund >Sweden > > > Jose C. Segovia. PhD Dpt. of Molecular and Cellular Biology CIEMAT Av. Complutense, 22 28040 Madrid SPAIN Tfn : 34 91 346 6484 Fax : 34 91 346 6393 E-mail : segovia@ciemat.es
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