Geoff,
We have sorted GFP expressing germ cells from 12.5 day old transgenic
embryos. The GFP expression was very high. The user sends you this
reference:
Development 126,1655-1664 (1999)
It may be a problem with your vector system. You do not mention if
you are looking at progenitor cells or entire bone marrow. Progenitor
cells are difficult to transduce (since they replicate seldom).
Lucy Brown
Beckman Research Institute/City of Hope
626-359-8111 X3306
lbrown@coh.org
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