Re: staining live yeast cells with hoechst stain

From: Ann Atzberger (Ann.Atzberger@EMBL-Heidelberg.de)
Date: Wed Jul 07 1999 - 05:17:44 EST

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>to whom it may concern
>the problem i have is that most people fix yeast cells before adding the
>hoechst stain to them for DAPI filter images of the nucleus , but i want to
>stain live yeast cells  as fixing cells bleaches the GFP fluorescence which i
>want to localize to the nuclear region. so i need to get a protocol for hoechst
>staining of LIVE yeast cells, without fixing them. i have tried adding hoechst
>to a liquid culture but the cells dont seem to take up the dye unless they are
>fixed. i would appreciate any suggestions with regard to this problem
>thanks
>roopa


Oops, sorry Roopa,

I realise after reading your message that dead cells aren't the issue.

regards
Ann

Next message: Marcelo B. Sztein, M.D.: "Flow Cytometer Operator/Laboratory Supervisor Position"
Previous message: Alberto Alvarez Barrientos: "Re: GFP and temperature dependance?"
Maybe in reply to: ROOPA ANDHARE: "staining live yeast cells with hoechst stain"
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