RE: Cy7-APC on 1991 Elite?

From: Tom Mc Closkey (thomasm@nshs.edu)
Date: Tue Jul 06 1999 - 13:55:33 EST


--- On Thu, 01 Jul 1999 15:12:30 -0400 (EDT)  James Weaver 301-594-5879 FAX 
301-594-3037 <WEAVER@cder.fda.gov> wrote:

>
>Does anyone know if the PMTs in a 1991 vintage Coulter Elite have
>sufficient far red sensitivity to 'see' antibodies labeled with Cy7-APC
>(PharRed et al) conjugates?

	I was able to detect [barely] CD8 buiotin/Pharred streptavidin on a 
stock Elite ESP using freshly isolated human lymphocytes.  The "dimmer" 
antigen s I tried were not resolvable.

Regards,
Tom



--------------------------------------------------------
Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
fax:  516-562-2866
7/6/99   11:57:49 AM
E-mail: thomasm@nshs.edu 
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