Craig, with isolated platelets we did some experiments using CFDA-AM for cytoplasmatic labelling of the cells. In my hands this worked really good and cells became very bright after cleavage of the AM ester. Unfortunately I donīt know whether loading of cells affects functional properties or not. Georg ------------------------------------------------------------------------ H.G. Kreysch Merck KGaA Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323 Frankfurter Str. 250 FAX: (49)6151 - 72 7398 D-64271 Darmstadt E-Mail: kreysch@merck.de
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