>We have used thiazol orange to stain our parasitised red blood cells and
>have been unable to separate the ring stage parasite from the trophozoite
>stage. Has anyone had success with this?
>
>Collette Hillier
>University of NSW
Collette,
We have been staining for Plasmodium in RBCs with hydroethidine and it
has been our experience that the rings stain less than the trophs. While
we have not sorted the specific populations, the percentages in the
different stages that we see by flow have correlated with the RBCs examined
and counted with a microscope. Please reference "Assessment of cell
cytotoxicity against the malarial parasite Plasmodium falciparum by flow
cytometry using hydroethidine" van der Heyde HC, et al. in Clin. Diag.
Lab. Immunol. 2(4):1417-1425, 1995.
Kathy
--------------------------------------------------------------
Kathy Schell
Supervisor, UWCCC Flow Cytometry Facility
600 Highland Ave. K4/535
Madison, WI 53792
Voice: 608-263-0313
e-mail: kschell@facstaff.wisc.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:39 EST