HI, Just today, I have someone trying the reagent described in Experimental Cell Research 238,498-502 (1998) Flow cytometry of Mitotic cells, HJ Anderson, et al. it looks ok so far.... DaveG. > >For M try MPM-2, DAKO. > >-----Original Message----- >From: owner-cytometry@flowcyt.cyto.purdue.edu >[mailto:owner-cytometry@flowcyt.cyto.purdue.edu] >Sent: Wednesday, June 23, 1999 12:55 PM >Subject: > > >Date sent: Wed, 23 Jun 1999 10:38:15 -0500 >From: Kevin Waddick <waddi002@tc.umn.edu> >Send reply to: Kevin G Waddick <waddi002@tc.umn.edu> >Subject: Cyclin B > > > Do any of you people out there know if a MoAb is available against >cyclin B or B1 that is conjugated to PE (or any fluorochrome other than >FITC that is suitable for FCM)? I am at a loss for where to look next >after checking out the sources of purified anti-cyclin B or B1 antibodies >listed in the Linscott's Directory without success. > Also, are there any strictly M phase-specific MoAb available other >than to p105? > I am asking these questions because I am attempting to verify the >results from a flow cytometric assay I have been developing through use of >independent cell cycle markers. I need to be able to separate cells >expressing cyclin B(1) into subsets through co-staining with other MoAbs >labeled with FITC. > I hope someone out there can help me with this! > >Dr. Kevin G. Waddick >Hughes Institute >2657 Patton Road >St. Paul, MN 55113 > > > > > Dave Gebhard--Director FACS Facility Albert Einstein College of Medicine Chanin Building 309 1300 Morris Park Avenue Bronx, New York 10461 718-430-2724/ 3573 FAX 718-430-8550
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