Greetings, We are attempting to use time of flight (pulse width) on a FACScan to accurately measure cell size on different cell lines. We are trying to avoid the sticky business of using forward scatter with bead standards to establish a curve. That method has its own set of problems. As FACScan users are aware, pulse width is only available on the three fluorescent channels. We initially tried using FDA to get a decent fluorescent signal for the pulse processor to derive the width signal. Our results did not correlate with known cell sizes. The trends were all wrong. Has anyone else successfully used the time of flight on a FACScan for such measurements? Thanks for your input. ------------------------------------------------ | Justin Fishbaugh | | University of Iowa | | Flow Cytometry Facility | | 48 EMRB | | Iowa City, IA 52242 | | | | justin-fishbaugh@uiowa.edu | | http://www.medicine.uiowa.edu/flowcytometry/ | ------------------------------------------------
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