Dear flowers, I am transfecting cells with GFP and when I try to do BRDU Immunefluouresnce with the HCl protocol, all DAPI and GFP staining dissappears... I tried twice the DNase method without any luck. Can you help? thanks a lot Adrian Senderowicz, M.D. DTP Clinical Trials Unit National Cancer Institute 10 Center Dr. MSC 1594 Bldg 10, rm 6N113 Bethesda, MD 20892 301-496-4119 301-480-7456(fax) sendero@helix.nih.gov
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