fluo4/fura red

From: David.McFarland@mcmail.vanderbilt.edu
Date: Wed Jun 09 1999 - 17:15:47 EST

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I  just tried fluo4/fura red for the first time and I saw a lot of crossover of
the the fluo4 into the fura red channel.  About a 10X increase over unstained
cells.  As all FACSCalibur users know, there is no way to directly compensate
FL3-FL1, so what now?  Is this just a matter of them adding way too much fluo4?
I'm open for suggestions.

David McFarland
Howard Hughes Medical Institute
Flow Cytometry Facility
Vanderbilt University Medical Center

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