Re: [Viability of lymphocytes.]

From: Cliff McArthur (cytocliff@netscape.net)
Date: Fri May 21 1999 - 09:52:31 EST


Mathi <mathi@meyerpharm.com> wrote:

Dear Flowcytometrist,

I am very confident that one or many of you out there have the
information I need. I want to know the maximum pressure which will
maintain  the maximum viability of activated mononeuclear cells in hig
pressure sorting or any other equivalant procedure.

Please send me your help.

All your help or datas will be received with gratitude and appreciation.

Thank you.

Mathi


Mathi,

I am sure you will hear from other MoFlo users as well on this.  We routinely
sort mononuclear cells at 60psi, 20,000+ events per second, all the time
maintaining viability.  Cells go back into culture, grow, proliferate, produce
cytokines just wonderfully, etc.

Cliff McArthur
UC San Francisco
415-476-6309

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