Re: autofluorescence

From: 'FR' Frank Radella II (fradella@u.washington.edu)
Date: Wed May 19 1999 - 15:40:21 EST


There are protocols using either trypan blue or crystal violet to quench
autofluoresence in fixed cells.  For starters look at Umino and Rennard,
AJRCCM 98 157:A810.

On Tue, 18 May 1999, bambam wrote:

> 
> Dear Flowers:
> 
> Does anyone out there know of any technique used to quench fluorescence?
> My organism seems to fluoresce in every wavelength.  Using Annexin-V-FITC
> and Tunel assays.  Any ideas would be great.
> 
> Thanks in advance.
> 
> Barb
> 
>   
> 
> 
> 



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