There are protocols using either trypan blue or crystal violet to quench autofluoresence in fixed cells. For starters look at Umino and Rennard, AJRCCM 98 157:A810. On Tue, 18 May 1999, bambam wrote: > > Dear Flowers: > > Does anyone out there know of any technique used to quench fluorescence? > My organism seems to fluoresce in every wavelength. Using Annexin-V-FITC > and Tunel assays. Any ideas would be great. > > Thanks in advance. > > Barb > > > > >
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