Sasha,
> Has anyone sorted mature mouse dendritic cells on a FACSVantage.
> Is a 70 µm nozzle OK or will I need a 100 or even bigger.
Yes, 70 micron is OK. We see no unusual losses on FACS II, FACStar or
MoFlo (the latter also runs at 60psi sheath pressure).
\ / < The Cytometry Laboratory
Frank Battye, \__/ <<<<< The Walter & Eliza Hall Institute
Dora Kaminaris,----------!!<<<<<<<< Victoria 3050, AUSTRALIA
Viki Lapatis and /!!\ <<<<< ph: 61_3_9345 2540 fax: 61_3_9347 0852
Jennie Parker o !! \ < in:: "facs_copy@wehi.edu.au"
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