Some weeks ago I asked imput about fluorochrome conjugation to antibodies. I thank very much to everyone who answered. Here a little summary of the answers. Lots of useful information regarding antibody conjugation can be found at Mario Roederer's website: http://www.drmr.com/abcon/ Kits for antibody conjugation are sold by Prozyme http://www.prozyme.com and by chromaprobe http://www.chromaprobe.com, but others suggest to use the chemicals sold by molecular probes http://www.probes.com. The kit from prozyme was described to conjugate 1 mg af antybody, to take only 2 hours from start to finish and to include everything you need. On the other hand it seems that PE conjugates are not quite good as commercially available ones, having higher background a not quite as strong a signal. Thank you again for answering Claudio Vallan =================================================== Claudio Vallan PhD Phone Lab: 031 / 632 88 76 FACS-LAB DKF Phone Office: 031 / 632 99 68 University of Bern E-Mail: vallan@dkf7.unibe.ch c/o Institute of Pathology Murtenstrasse 31 Insel hosptial area only: 3010 Bern Beeper: 181 67 59 ===================================================
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