First I want to thank you all for your responses. I got about 22 responses regarding my question about possible upgrades from DOS Elite software to the Windows Expo software, and also answering the question about the Windows WIN-FCM software for the FACScan. 1) One person suggested that having a lone Mac in the department was not a big deal, as it is easy to network the computer to an NT environment. That wasn't our experience, but that may have been a problem with our computer support people more than with the system. The files are compatible witn WIN-MDI and other related software analysis packages which have all been mentioned. The advantage with Cellquest is that BD would cover it in service, and I don't know how BD would react to WIN-FCS on their system. 2) A couple of people suggested the Cicero system from Cytomation, which runs on a Windows NT environment, and they seem happy with it (though one of the responses on that was from Cytomation). 3) Several people noted that both Win-FCM and Expo are manufactured and programmed from the same company, Applied Cytometry Systems, so if both BD and Beckman-Coulter (BC?) machines are in our department, there will be only one interface (more or less, the Expo software is a more current version as I understand it). 4) One person remarked that the Expo software didn't do anything more than the DOS software, so they didn't buy it after demonstrating it. That's probably true, but at least it now does everything that the DOS software does. Last year when I asked the question, some people said that it didn't run the autoclone, and the reply I think you all received said that it worked fine. 5)The only problem people seemed to mention with the WIN-FCM was that the "Dongle" connected to the parallel port seemed to go bad upon occasion. ACS replaced it without question, but I imagine that that is a problem that could be frustrating. Conclusion: The windows packages seem to work okay, though some thought Cellquest works better, though that could be a Mac prejucice :-). The people who use them seem to be happy with them. They are not cheap, that is, it isn't like buying a copy of MS Word or something like that, but they seem to do what they are advertised as doing. There are about 10 users of WIN-FCM in the USA, if my information is correct, and I don't have a good number of Expo users. We will probably get the Expo software if we have the money for it, sooner or later I am going to be left in the dust in the DOS environment. For the FACScan, I am not making the final decision, but we will have to upgrade from the HP computer, even though we could use the 1972 solution for the Y2K problem. Sorry if this note was long winded, but I hope that this summary is useful. Doug - {-----------------------------------------------------------------------} Douglas Smoot Immune Cell Biology Program Naval Medical Research Center 8901 Wisconsin Avenue Bethesda, MD 20889-5607 voice: 301-295-6272 fax: 301-295-0376
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