At 10:35 5/3/99 +1000, A.Mitchell@unsw.EDU.AU wrote: > >Dear flowers, > >I am posting this request on behalf of a colleague. It is not specifically >flow related, but could benefit from input from the list. > >The investigator is looking for an anti mouse kappa chain antibody, the >purpose is to show pairing of heavy and light chains in phage antibody >display. They have tried a couple of sources but have had concerns about >the quality of antibodies. Can anyone suggest suppliers? Andrew: Yes, I have a suggestion. Ware,CF; Reade,JL; Der,LC. 1984. A rat anti-mouse kappa chain specific monoclonal antibody, 187.1.10: purification, immunochemical properties and its utility as a general second-antibody reagent. J. Immunol. Methods. 74: 93-104 it describes the 187 Rat anti mouse kappa (constant) antibogy. Given that the majority of mouse Ig's are kappa this is a great reagent. It is very specific and does not recognize lamda chains (OKT3) for example. it is available from ATCC. Hope this helps, David ============================= David L. Haviland, Ph.D. Asst. Prof. Immunology University of Texas - Houston, H.S.C. Institute of Molecular Medicine 2121 W. Holcombe Blvd. Houston, TX 77030 Internet:"dhavilan@imm2.imm.uth.tmc.edu" Voice: 713.500.2413 FAX: 713.500.2424 ------------------------------------------------------ The hardness of butter is directly proportional to the softness of the bread. =============================
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