Hi Greg Students and indeed investigators new to analysing and interpreting list-mode data shouldn't dismiss the expertise of experienced operators offhand. Gating data is a powerful tool. I would start though with a simple package like Cellquest for the mac. Almost everyone I have talked to has found Flow JO not very intuitive and don't care to use it, myself included.I also felt it ran very sluggishly on a Powermac-maybe it does better on a G3. Marcia Woda Manager, FACS Shared Instrument Facility Univ. of Mass Medical School Worcester, Ma. 01655
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