Hi! I'd like to use FACS to analyze the DNA content of bacterial cells in a population (the question is if the bacterium we're studying segregates daughter cells carrying only one of the chromosomes under starvation conditions. These cells should be detectable as a population with dramatically reduced DNA content.) I was wondering if there are protocolls out there for staining bacteria for FACS analysis. Preferably a stain that does not overlap with the emisssion spectrum of GFP. Also the FACS that is available to me does not have a UV laser, i.e. DAPI will not work. I'd appreciate any info/protocols you may have to offer! Thanks! Arne ____________________________________________ Arne Rietsch Dept. of Microbiology and Molecular Genetics Harvard Medical School 200 Longwood Ave., Boston, MA 02115 Phone:(617) 432-0832 FAX: (617) 738-7664
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