Dear Flowers... We've been working with an investigator at our facility whose specimens are driving us a little bonkers. She is trying to do cell cycle analysis (via PI staining) of ATCC HTB 17 cells which ATCC describes as a hypotriploid glioblastoma, astrocytoma, grade III, human. A karyotype of her cell line showed 6 different populations with both multiple trisomys and numerous translocations appearing at different locations in each population. If anyone has had experience working with this cell line or something similar, or if anyone just has some suggestions, I would love to get a little advice on how to accurately look at their cell cycle analysis. Many thanks in advance and best wishes. Matt Morrow, MS, MT (ASCP) Flow Cytometry Core Research Laboratory H. Lee Moffitt Cancer Center and Research Institute (813) 972-8400 Ext. 2005 Morrowmr@moffitt.usf.edu
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