> --On Fri, Apr 23, 1999 9:45 AM +0000 Rita Cavaleiro > <ritacavaleiro@hotmail.com> wrote: > > > we obtain, unexpectedly, a lot of positive cells for annexin in > > unstimulated human PBMCs in culture for 24 hours. I am not sure if > > this high level represents unspecific labelling because there is no > > wash after the adition of annexin. None of the protocols use this > > wash. Could anyone tell me if there is any problem in doing this wash? ............ What is the best positive control to induce apotposis in > > human PBMCs and in what concentrations ?. I tried Dexamethasone but it > > did not work. > > a 'background' after 24h in vitro culture of about 7% is normally found (if incubated in 'optimal' conditions, e.g. with FCS 5%). A possibility for positive control is gamma-irradiation. More data can be found in Cytometry 1997; 29: 242-9. Jan Philippé e-mail: jan.philippe@rug.ac.be
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:25 EST