Dear Flow-people, I have two questions that I would really appreciate some help with: 1. Is there anything I can do to reduce the 'stickiness' of my streptavidin-PE/Cy5 conjugate? Including human IgG as an Fc block helped a little, but on lymphocytes (human) I am still getting a tail on my negative population, and on monocytes there is almost a full decade shift in fluorescence of the entire population. Is this normal?? The conjugate is from Rockland. 2. Does anyone know of a source of antibodies (monoclonal or polyclonal) against any mouse chemokine receptors?? Thanking you in advance, Lisa Gale ___________________________________________ Lisa Gale Chemokine Biology Laboratory Department of Microbiology and Immunology University of Adelaide, SA, 5005, Australia TEL: 61-8-303 4630 FAX: 61-8-303 4362 Email: lgale@microb.adelaide.edu.au
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