I've been using streptavidin-allophycocyanin to label biotinylated primary or secondary antibodies, in a second step after washing off unbound biotinylated antibody. Has anybody applied streptaviding-conjugated fluorophores at the same time as the biotinylated antibody? It seems to me it should work fine, and it certainly would save time.. Thanks!! ......Ioana Ioana Sonea, DMV, PhD, Adjunct Assistant Professor, Dept. Biomedical Sciences, Iowa State University, Ames, IA 50011 Tel: 515 294 6875 Fax: 515 294 2315 E-mail: ioana@iastate.edu
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