We are trying to stain for intracellular TGF-beta 1 in T cells, so far without much success, although we can clearly detect other cytokines intracellularly and also find TGF-beta in the supernatants of the same cells. Has anybody tried intracellular staining of TGF-beta either for flow-cytometry or fluorescence microscope (negative results may also be helpful)? Which antibodies (company, clone) could be suitable for detecting the latent, intracellular form of TGF-beta 1? Any comment would be helpful Alex Alexander Scheffold Deutsches Rheumaforschungszentrum Berlin Hannoversche Str. 27 10115 Berlin phone +49 030 2851-8919/64 FAX +49 030 2851-8910 e-mail: scheffold@drfz.de
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