to all those flowers out there, I have some questions.......does anyone know of a supplier that has a selection of guinea pig reagents??? I am currently trying to do intracellular staining of gpIFN-g in CHOK1 cells and have not had much luck with cross reactivity of human IFN-G, another one would be gpIL-2. Another question I have about staining procedures: what is the range of Ab concentration used in staining (I used 0.06ug/10^6 cells as recommended, but I thought this was low??)? and when preparing the cells for staining is it necessary to plug the cells before fixing so that the normally secreted cytokine remains inside or is this accomplished during fixing??? please I need some insight!!! mary Maryann Giel Aquila Biopharmaceuticals mgiel@aquilabio.com
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