Hello Flowers, I would like to determine the oxidative burst of murine macrophages and PMN by flow cytometry. In literature and the Orpegen test 123 dihydrorhodamine is used, but there is no concentration mentioned. Could anyone give me advice at which concentration to use it and how to handle it ( I think it is extremely sensitive to oxidation-- do I have to use inert gas?) I would appreciate any comment on this subject. Thanks, Martin Lehner University of Konstanz Germany lehner@tycho.gk-biochem.uni-konstanz.de
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