Neutrophils and FCM

From: Ross Longley (Longley@HBOI.edu)
Date: Thu Apr 08 1999 - 13:33:27 EST


April 8, 1999.

Hello - Does anyone know of a good flow cytometry marker or antibody to
identify neutrophils in a population of human peripheral blood leukocytes?
CD16 has been suggested, but I believe this antigen is also expressed on NK
cells.  Any thoughts on this would be greatly appreciated.  Also, methods by
which one can isolate reasonably pure neutrophil populations?

Color me "neutrophil naive".

Ross E. Longley, Ph.D.




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