Hi Everyone, I and a co-worker would like to try our own hand at conjugating Cy7 to APC and then to a monoclonal antibody. I am aware of a protocol or two out there detailing just this process. What I am hoping for is this: any chance any of you have actually done this with success and would be willing to give me a head start? It would be wonderful if you could let me know what combinations of Cy7 and APC (quantities) worked best for you, from whom you obtained the Cy7 and APC (or did you harvest the APC yourself?), and any other details or tips you think might help us out. I send huge amounts of thanks in advance. Cliff McArthur, Specialist Department of Medicine Division of Infectious Diseases University of California at San Francisco 415-476-6309 415-502-6860 ____________________________________________________________________ Get your own FREE, personal Netscape WebMail account today at http://webmail.netscape.com.
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