Dear John, We usually find Large B-cell Lymphoma cells on the CD45 x SS gate to the left of the normal lymphocytes, indicating that they are CD45 dim. We also characteristically find that they have unusually low side scatter and ofter appear "crushed" against the bottom of the histogram, indicating very low side scatter. We have found Large B-cell Lymphomas to be very fragile tumors and retrieve the best cells when we process the specimen immediately after receipt and do not postpone the analysis in any way. Although we are successful in finding the tumor cells the vast majority of the time, occasionally we only isolate normal lymphocytes despite the histology indicating Large B-cell Lymphoma. This indicates to us that the cells have apparentlly died and disintegrated before we could isolate them. This is a difficult tumor to analyze by flow, and I wish you the best of success! Cynthia B. Aller, Ph.D. Director of Flow Cytometry Medical Science Laboratories Wauwatosa, WI 53226 (414) 256-1480 caller@execpc.com
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