When I balanced the costs of : 1- making my own HBSS from powder and filter sterilizing (autoclaving glass is not an option here for several reasons). 2- using an 8x sheath fluid from Reise (I hear it is very good, but must be prepared and filtered....filtering is also a challenge at times at my company). 3- using an already prepared and sterilized 1 x HBSS w/out Ca, Mg, in 1 liter bottles. I chose option # 3.....some clinical areas also have available to them clinical saline at a good price. That is also not available to us, but perhaps to you, and there are other options as well of which the rest of the flow world will tell you! P. Echeagaray SRI-Serquest Frederick MD, USA > -----Original Message----- > From: Cliff McArthur [SMTP:cytocliff@netscape.net] > Sent: Friday, March 26, 1999 6:51 PM > To: Cytometry Mailing List > Subject: Viable cell sorting sheath fluid > > > Hi Everyone, > > I could use a tad bit o' help here: > > I've been using with great success for some time now Criterion Sciences > Diluent 2 as a sheath fluid for viable cell sorting. Well, come time to > order > some more, I find that VWR Scientific (my supplier) was no longer carrying > it. > I cannot find Criterion Sciences on the Web either. I'm a little > baffled. > They and their "diluent 2" still exist, don't they? Anyone know? > > This seems like a good time to ask: what is the commercial sheath fluid > (again, for viable cell sorting) of choice nowadays, that you all use? > Could > you let me know who, what and roughly how much? I am preparing my own for > the > time being, but I much prefer the commercial sheath as it leads to cramps > in > my day of lesser frequency. > > Many thanks, > Cliff McArthur, MT(ASCP), Specialist > Department of Medicine > Division of Infectious Diseases > University of California at San Francisco > 415-476-6309 > 415-502-6860 > > ____________________________________________________________________ > Get your own FREE, personal Netscape WebMail account today at > http://webmail.netscape.com.
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