Multi-color assays

From: Gregor Rothe (gregor.rothe@klinik.uni-regensburg.de)
Date: Mon Mar 29 1999 - 03:42:23 EST


Dear all,

In a process of writing a review on multi-color techniques used for 
flow cytometry or microscopy I would like to cite the most early 
publications on multi-color excitation with a single light source. 

My guess is that in flow cytometry splitting of a single laser line 
for subsequent pumping of a laser dye would be the earliest 
technique. Or have people been using a laser in a multi-line mode, 
previously?

Regarding microscopy, I remember that dual bandpass filters or double 
reflection dichroits were made available around the end of the 80s. I 
remember to have seen data from David Marcus, Omega Opticals, around 
that time. Is there any early reference for the use of such filters 
or dichroits in any type of instuments?

Thanks for any help,

Gregor Rothe

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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
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D-93053 Regensburg, Germany

Tel. +49 (941) 944-6204
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E-mail: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE
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