Has anyone out there in flowland looked at cell cycle in pig myoblasts. We are having difficulty getting good staining. We have played around with the detergent concentration in Vindelov's PI, but can't get totally free nuclei, and the DNA appears to be leaking out of the cells. Also clumping is a major problem. These are adherent cells that have been trypsinized then "bathed" in 0.53mM EDTA. Any suggestions would be welcome!! Thanks in advance, Kathy Kathy Ragheb, MT(ASCP) Purdue University Cytometry Laboratories HANS B050, West Lafayette, IN. 47907 ph:(765)494-0757 fax:(765)494-0517 EMAIL:ragheb@flowcyt.cyto.purdue.edu WEB:http://www.cyto.purdue.edu
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