We just had a used FACStar+ installed and now we're in the market for a used recirculating water bath. (Dem new ones ain't cheap!) I've come across two flavors. One just recirculates water around the sample and collection tubes. The other is a real bath that you also place the sheath tank in. Do I really need to keep the sheath at temp? Also, we're considering a Time Zero module from Cytek for Calcium flux experiments. I invite any comments on this system. We also have a FACSCalibur and I'm wondering how well Cytek's 20L Cubitainer sheath and waste system works. Is it worth the $2450 to not have to empty/fill the fluids every couple of hours? I look forward to your replies. David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center
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