Intracellular staining for chemokines

From: gborsel@box1.tin.it
Date: Thu Mar 25 1999 - 19:11:17 EST

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Dear cytometrists,

does anyone have experience in intracellular staining for chemokines? 
We routinely stain T lymphocytes  for intracellular IL-4 and IFN-g, 
but have thus far been unable to stain for intracellular chemokines 
(MIP-1a, RANTES and MCP-1 are the ones we tried) using R&D 
antibodies.
Any suggestions would be greatly appreciated.

Thanks 

Giovanna Borsellino
Neuroimmunology lab
IRCCS S.Lucia
Rome

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