-----Original Message----- From: Bill Hyun [mailto:hyun@cc.ucsf.edu] Sent: Wednesday, 10 March 1999 2:57 To: Robert Ashcroft, PhD Subject: Re: the truth is out there,... all help gratefully received! Bob, Here's one...call Ron Jensen (415)476-3383...he did all the testing of radiation-induced glycophorin-A mutations on RBCs as a test for radiation dosimetry on Chernobyl survivors. * Bill Hyun >A quiet request from Pbob, suffering from paperptosis. Call it what you >like! > >I am writing a review of the use of flow cytometry analysis in truly rare >cell enumeration. >I am not looking at analysis only and not sorting, except where it's used >essentially for verification of the counting, eg to ensure that the counted >cell was actually the target cell. > >I define truly rare cell enumeration as counting cells occurring at >frequencies at or below one "target" cell per thousand abundant cells. I >might consider one per 100, but presently I prefer not to, as I consider 1% >pretty trivial. I am open to argument though. > >When I was a mainstream academic back in the 1980s, people such as Jim >Leary, Jan Visser, Paul Horan, Ken Bauer, Jim Cupp and Betsy Ohlsson-Wilhelm >were the pace-setters in these areas. I guess that there are others who have >now joined them in this area, especially given the advances in foetal cell >and stem cell research, including the recent revelations about CNS stem >cells. > >If you have written or know of a paper where flow measurements were used to >count such cells within my definition, would you please let me know about >it. Attached files are welcome! > >I prefer that you answer off-List, but should anyone want a copy of the >resultant paper-list, I will forward it upon request. Probably about the end >of the month, when my son finishes rowing season! > >I plan to cover as wide an area of usage as exists out there, whether it be >transfection studies in eukaryotes or prokaryotes, bacterial mutants, >leukocytes in packed red cells, foetal bleeding, minimum residual disease, >diagnosis of metastasis, developmental-cell trafficking, monitoring >treatment regimes, clinical or fundamental, medical or biological, etc, etc. >Whatever. > >It will cover use of extrinsic and intrinsic markers, a variety of laser >excitations and the benefits of multi-colour-epitope labelling vs single >fluorochromes, doublet discrimination, acquisition rates, sensitivity etc. > >I am wildly out of touch with the Journal Literature, but I hope that y'all >out there can assist. > >Thanks for your time and possible input. >Pbob ***************************** William Hyun, Director Laboratory for Cell Analysis UCSF Cancer Center, Room S129 San Francisco, CA 94143-0808 Office (415)476-2632 Lab (415)476-2631 FAX (415)476-8218 email hyun@cc.ucsf.edu Deliveries: 2340 Sutter Street, Room S129 San Francisco, CA 94115
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