Diane It sounds like you are seeing endoreduplicating cells. There is a paper in the most recent Cytometry from the Jaccoberger group about these and how to separate the 4N G1 from the 4N G2M. Larry Arnold At 08:53 AM 3/17/99 -0500, you wrote: > >Dear colleagues, > >I have a B-D FACScan and routinely do PI staining for cell cycle analysis. >I gate on width and area (doublet discrimination). Does this guarantee >exclusion of doublets? I can clearly gate out doublets that appear to be 4N >DNA content with a large pulse width signal, but I also often see greater >than 4N events that do not have a large width pulse. Are these truly >4N >cells? The cell lines I use are adherent human tumor and fibroblast cell >lines. Any input would be appreciated. > >Diane Sharp >diane.m.sharp@dupontpharma.com >
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