We are looking for down regulation of platelet CD42 after TRAP or ADP activation but we haven't see anything using the following protocol 5µl of activating agent ADP or TRAP 50µM + 5µl of citrate whole blood 10 or 30 minutes incubation at 20°c or 37°c After activation, we add 5µl of CD61 PerCP (BD) and 5µl CD42b (Pharmingen) After 10 minutes we stop the reaction by fixation with formaldehyde Do someone have any experience in that field or a protocol for such experiment? The goal of our study is to investigate a rare Bernard Soulier variant disease. Thank you for considering my request -- DELVILLE Jean-Pierre MS Laboratoire d'hematologie Route de Lennik,808 1070 Brussels BELGIUM Tel 32 2 555 39 48 Fax 32 2 555 44 99 E-mail jdelvill@ulb.ac.be
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