Greetings again..... We are attempting to assess apoptosis in a transfected cell line in which we can not use annexin (the cells are far too adherent, and by the time they are harvested, annexin binds with an equally high affinity to all cells). We would like to use mitochondrial membrane potential, but need an indicator that does not obscure our GFP signal (i.e. excited by the 633 nm diode on our Calibur). Previously I had seen people use DiOC6(5), but Molecular Probes no longer offers this product. Molecular Probes suggested we try DiS3(5), and I wondered if anyone had used this indicator with any success, or if there were any other suggestions for 633 excitable, >670 emitting indicators?? Thanks in advance for any replies, Keith Bahjat Graduate Assistant University of Florida College of Medicine kbahjat@ufl.edu
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