SYDFLOW The Sydney Flow Cytometry Group (AUSTRALIA) The First Sydflow Meeting for 1999 will be at Prince of Wales Hospital Randwick on Monday the 29th March 1999, at 6pm in the Edmund Blackett Lecture Theatre. (Kindly sponsored by Becton Dickinson) The Agenda will be : 6:00 - 6:30 Refreshments 6:30 - 6:45 Physical proximity of Platelet Surface Proteins: Analysis using FRET. Janette Burgess PhD. Centre for Thrombosis and Vascular Research UNSW. Department of Haematology Prince of Wales Hospital An analysis of the relationship of glycoprotein 1b( and protein disulphide isomerase on the platelet surface membrane. Fluorescence Resonance Energy Transfer and the binding of a monoclonal antibody panel were used to characterise the close proximity of these platelet plasma membrane proteins. 6:45 - 7:00 Monitoring Apoptosis initiated in Cord Blood CD34+ Haematopoietic Cells in Suspension Culture by Flow Cytometry. Helen Tao PhD Centre for Thrombosis and Vascular Research UNSW. Department of Haematology Prince of Wales Hospital. Utilising serum-free suspension culture system, purified cord blood CD34+ cells are induced to proliferate and differentiate into myeloid, erythroid and megakaryocytic cells in the presence of appropriate growth factors. Apoptosis in these haematopoietic cells is monitored by flow cytometry in our laboratory. This provides useful information for studying the in vitro behaviour of cytokine mediated haematopoietic cells, when investigating the effects of various stimulants and inhibitors of haematopoiesis. 7:00 -7:15 Administration of anti-CD3 Monoclonal Antibodies to Pigs. A/Prof.Bernie Tuch Department of Endocrinology Prince of Wales Hospital Anti-CD3 monoclonal antibody is used in humans as an immunosuppressive agent. Whether it can be used in pigs for this purpose is unknown. The aim of the current experiment was to address the safety and efficacy of giving these antibodies to diabetic pigs transplanted with insulin producing fetal pig cells. 7:15 - 7:30 Break. 7:30 - 7:45 The Use of Flow Cytometry in the Assessment of Engraftment of Cytokine Expanded Cord Blood Stem Cells Julie Stevenson Cord Blood Research Program ,Children's Cancer Research Institute Sydney Children's Hospital Randwick. The NON-SCID mouse model provides a technique for the assessment of the engraftment capacity of the cytokine-expanded human cord blood stem cells. We have established this model in our laboratory and developed a variety of techniques to detect engrafted human cells in a mouse background. 7:45 - 8:00 Partial Differentiation and Expression of B7-1 by Human Leukaemic Cells Cultured in GM-CSF, IL-3 and IL-6. Christine Hicks PhD Department of Haematology Prince of Wales Hospital. Leukaemic blasts from AML patients were cultured in the prescence of GM-csf, IL-3 and IL-6. The cells were partially differentiated including the upregulation of CD80(B7) important for immune stimulation. These blasts may have a potential role as immunogenic modulators in AML. Jenny Bryant Flow Cytometry Laboratory Australian Red Cross Blood Service - NSW 153 Clarence St Sydney, NSW 2000, AUSTRALIA .._|\ Ph: 61 2 9229 4341 / \ FAX: 61 2 9229 4372 \_.-._/<<<<<<<<< Email: jbryant@arcbs.redcross.org.au v
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