You wrote Has anyone used anything other than PI to do viability staining on the FLOW? 7-AAD? I have a researcher using PI and AO and they co-stain ALL the cells, virtually 100%, in what they believe to be live samples of Chondrocytes. Any hints? Does the collagenase used to break up the matrix of the cartilage cause permeabilization? I'm kind of at a loss to explain to him what is happening. Any better dye for this application? Thanks in advance! Susan DeMaggio, MS BSMT(ASCP)QCym Resource Manager Optical Biology Shared Resource University of California, Irvine Irvine, CA 92697-2275 phone 949-824-4110 fax 949-824-3571 Dear Susan, I routinely use the 7AAD instead of PI... It works weel exept that the scale of fluorescence is a bit less large. The emisson max is at 647 nm and the MW 1270. Pi has a MW of 668. The diffrence can explain some discripency in using the two dyes. But at the main value.... 7 AAD is very usefull. Patrice Dr. Petit Patrice X. Institut Cochin de Génétique Moléculaire INSERM U129 - CHU Cochin Port-Royal 24, rue du Faubourg Saint-Jacques F-75014 Paris, France. Tel: 33 01 44 41 24 11 Fax: 33 01 44 41 24 21 E-mail: pxpetit@icgm.cochin.inserm.fr
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