Dear all, Wayne Green wondeed why his recovery was low when sorting C. elegans. Wayne was using a Vantage with a 100 micron tip (no Macro Sort). The embryos are 50x30 microns size. I feel that the size of the embryos is the problem. As a rule of thumb I believe that the tip diameter should be 3 or 4 times the size of the largest particle being sorted to achieve efficient recovery, i.e. 150-200 microns in this case. Yours sincerely, Nigel Blackhall, Experimental Officer, Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK Tel +44 115 9515151 ext 18501 Fax +44 115 9513298
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